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目的探讨黄芪多糖对胃癌细胞SCG-7901上清液中培养的树突状细胞(Dendritic cells,DC)分化成熟及功能的影响,分析黄芪多糖抗癌的作用机制。方法将人外周血分离的单核细胞加入含重组人粒细胞-巨噬细胞集落刺激因子(rhGMCSF)和重组人白细胞介素的培养液中,随机分为空白组(以RPMI1640培养液培养)、干预组(以黄芪多糖干预后的胃癌细胞上清液培养)、对照组(以胃癌细胞上清液培养)。混合培养48h后,显微镜下观察DC成熟过程中的形态学变化,流式细胞术检测DC的表型(CD40、CD80),混合同种淋巴细胞增殖反应(Mixed lymphocyte reaction,MLR)检测DC的增殖效应。结果与对照组比较,空白组和干预组DC的CD40和CD80的表达均明显增加(P<0.05),刺激同种淋巴细胞增殖效应明显增强(P<0.05);干预组DC CD40和CD80的表达阳性率及刺激同种淋巴细胞增殖效应均明显低于空白组(P<0.05)。结论在体外,黄芪多糖可有效对抗胃癌细胞上清液引起的对DC分化成熟和功能的抑制。
Objective To investigate the effect of astragalus polysaccharides on differentiation and function of dendritic cells (DCs) cultured in gastric cancer cell supernatant SCG-7901, and to analyze its anti-cancer mechanism. Methods Monocytes isolated from human peripheral blood were added into the culture medium containing rhGMCSF and recombinant human interleukin and then randomly divided into blank group (cultured in RPMI1640 culture medium) The intervention group (cultured with gastric cancer cell supernatant after intervention of Astragalus polysaccharide), the control group (cultured with gastric cancer cell supernatant). After 48 hours of mixed culture, the morphological changes of DCs were observed under the microscope. The phenotypes of DCs (CD40, CD80) were detected by flow cytometry. The proliferation of DCs was detected by mixed lymphocyte reaction (MLR) effect. Results Compared with the control group, the expressions of CD40 and CD80 in the blank group and the intervention group were significantly increased (P <0.05), and the proliferation of allogeneic lymphocytes was significantly increased (P <0.05). The expressions of CD40 and CD80 The positive rate and the proliferation of allogeneic lymphocytes were significantly lower than those in the blank group (P <0.05). Conclusion In vitro, Astragalus polysaccharide can effectively inhibit the maturation and function inhibition of DC differentiation induced by gastric cancer cell supernatant.