论文部分内容阅读
以阔叶猕猴桃(A ctinidia latifolia M err.)叶片为外植体,通过器官发生途径诱导形成不定芽,探讨了不同激素组合、暗培养时间以及不同浓度的蔗糖对叶片器官发生和植株再生的影响。结果表明,BW +0.1μm ol/L N AA +5μm ol/L Zeatin 附加20g/L 蔗糖,先进行21d 暗培养然后转到光下培养效果最好,6周后不定芽再生频率达91.67%,平均每个外植体再生芽数6.87个。再生芽生根良好,生根率可达100%。首次报道了阔叶猕猴桃的器官发生和植株再生,建立了叶片的高效再生体系,为今后的遗传转化研究奠定了基础。
Adventitious buds were induced by organogenesis using leaves of A ctinidia latifolia M err. As explants. The effects of different hormone combinations, dark culture time and different concentrations of sucrose on the organogenesis and plant regeneration of leaves were discussed . The results showed that 20g / L sucrose was added to BW + 0.1μm ol / LN AA + 5μm ol / L Zeatin, and the best effect was obtained after 21 days of dark culture and then transferred to light. After 6 weeks, the regeneration frequency of adventitious buds reached 91.67% The number of regenerated buds per explant was 6.87. Regeneration shoots well rooting rate up to 100%. The organogenesis and plant regeneration of broad-leaved kiwifruit were reported for the first time, and the efficient regeneration system of the leaves was established, which laid the foundation for the future research on genetic transformation.