建立了化妆品中硫柳汞和苯基汞的高效液相色谱分离,电感耦合等离子体质谱法(ICP-MS)测定的检测方法。方法采用SB C18反相色谱柱(4.6×150mm,5μm)分离,流动相为甲醇-60 mmol/L乙酸铵溶液(含0.1%L-半胱氨酸),经过梯度洗脱,ICP-MS测定组份中的202Hg,以保留时间定性,外标法定量。结果表明,在此条件下,硫柳汞和苯基汞在0～100μg/L范围内线性良好,两种有机汞的检出限分别为5.76μg/L和4.41μg/L,不同类型化妆品中硫柳汞和苯基汞的平均回收率在82.0%～118.7%范围内。方法适用于化妆品中硫柳汞和苯基汞的测定。 A method for the determination of thimerosal and phenylmercury in cosmetics by high performance liquid chromatography and inductively coupled plasma mass spectrometry (ICP-MS) was established. The method was separated on a SB C18 reversed-phase column (4.6 × 150 mm, 5 μm) using methanol-60 mmol / L ammonium acetate solution (containing 0.1% L-cysteine) as mobile phase and gradient elution. Components in the 202Hg, to retain the time qualitative, external standard quantitative. The results showed that under these conditions, thimerosal and phenylmercury had a good linearity in the range of 0 ~ 100μg / L, the detection limits of the two organic mercury were 5.76μg / L and 4.41μg / L, respectively. Thiomersal and The average recoveries of phenylmercury ranged from 82.0% to 118.7%. The method is suitable for the determination of thimerosal and phenylmercury in cosmetics.