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光敏色素在植物的种子萌发、幼苗去黄化、叶片开张、茎伸长、开花等发育过程中具有重要调节作用。本研究根据玉米基因组数据库设计引物,获得ZmPHYC1和ZmPHYC2基因序列,分析其编码的氨基酸序列和结构域发现,6个不同光温敏感性玉米品种中光敏色素C基因序列未出现大片段的序列插入或缺失,仅发现少量SNP;ZmPHYC1与ZmPHYC2蛋白中含有1个GAF结构域、1个Phytochrome结构域、2个PAS结构域、1个His Kinase A结构域和1个类似组氨酸激酶的ATP激酶结构域;遗传进化树分析表明,单子叶和双子叶植物明显聚为不同的类别,并且玉米和高粱之间的同源性要高于玉米中PHYC1与PHYC2之间的同源性。利用实时荧光定量PCR技术分析ZmPHYC1和ZmPHYC2的表达模式发现,在光暗处理条件下,两个基因在不同品种间及各组织部位中表达量无显著差异。
Phytochromes play an important regulatory role in plant seed germination, seedling etiolation, leaf opening, stem elongation, flowering and other developmental processes. In this study, primers were designed based on maize genomic database to obtain ZmPHYC1 and ZmPHYC2 gene sequences. The amino acid sequences and domains of ZmPHYC1 and ZmPHYC2 were analyzed. No significant insertions were found in the phytochrome C gene sequences of 6 different light-temperature-sensitive maize cultivars And only a few SNPs were found. The ZmPHYC1 and ZmPHYC2 proteins contained one GAF domain, one Phytochrome domain, two PAS domains, one His Kinase A domain and one His kinase kinase-like ATP kinase Analysis of the phylogenetic tree showed that monocotyledonous and dicotyledons apparently clustered into different categories, and the homology between maize and sorghum was higher than that between PHYC1 and PHYC2 in maize. The expression patterns of ZmPHYC1 and ZmPHYC2 were analyzed by real-time fluorescence quantitative PCR. The results showed that there was no significant difference in the expression levels of the two genes between different varieties and tissues under light and dark conditions.