目的评价经体外预处理、启动免疫保护功能的人胎儿骨髓间充质干细胞(hfBMSCs)的潜在自身成瘤性。方法采用流式细胞术检测细胞表型及细胞周期,染色体核型分析检测遗传稳定性,裸鼠皮下接种检测体内成瘤性。结果体外预处理的hfBMSCs呈正常间充质干细胞表型,可通过阻滞细胞周期于G0-G1期抑制hfBMSCs生长增殖(P<0.05),对染色体核型无影响,不具备体内成瘤性。结论经体外预处理的人胎儿骨髓间充质干细胞不具有自身成瘤性。 Objective To evaluate the potential self-tumorigenicity of human fetal mesenchymal stem cells (hfBMSCs) that have been pre-treated and immunostimulated. Methods Flow cytometry was used to detect cell phenotype and cell cycle. Chromosomal karyotype analysis was used to detect genetic stability. Nude mice were inoculated subcutaneously to detect tumorigenicity in vivo. Results The hfBMSCs pretreated in vitro showed the phenotype of normal mesenchymal stem cells, which could inhibit the growth and proliferation of hfBMSCs at the G0-G1 phase (P <0.05), and had no effect on the karyotype of the hfBMSCs and did not possess in vivo tumorigenicity. Conclusion Human fetal BMSCs pretreated in vitro do not have their own tumorigenicity.