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目的 :观察胸腺肽α1(Tα1)和α干扰素 (IFNα)对健康新生儿和成人单核细胞分泌三种C -C趋化因子的调节作用 ,探讨二者用于阻断艾滋病病毒 (HIV)母婴传播的可能性及其机制。方法 :Ficoll密度梯度离心、贴附法分离健康新生儿和成人单核细胞 ,FITCmAbCD14鉴定 ,与 1μg/ml的Tα1和 /或 5 0 0U/ml的IFNα共培养 3d后 ,ELISA法分别检测培养上清中的正常T细胞表达和分泌因子 (RANTES)、巨噬细胞炎症蛋白 1α(MIP - 1)和MIP - 1β。结果 :14例健康新生儿和 17例正常成年人单核细胞分泌RANTES、MIP - 1α、MIP -1β的浓度分别为 310± 90、2 310± 6 70、182 0± 75 0和 75 0± 2 10、2 5 80± 12 80、1910± 4 6 0 pg/ml。Tα1和 /或IFNα可明显上调单核细胞分泌的RANTES ,二者联用并有协同作用 ;MIP - 1α可被Tα1调高 ,新生儿单核细胞的MIP - 1β可被IFNα调高。上述处理对新生儿单核细胞的调节作用比对成人更明显。结论 :新生儿单核细胞分泌RANTES和MIP - 1α的能力明显低于成人。Tα1和 /或IFNα能上调新生儿和成人单核细胞分泌的三种C -C趋化因子 ,有可能降低新生儿单核细胞对HIV感染的易感性。
OBJECTIVE: To investigate the regulatory effect of thymosin α1 (Tα1) and interferon α (IFNα) on the secretion of three C-C chemokines in healthy neonatal and adult mononuclear cells The possibility of infant transmission and its mechanism. Methods Ficoll density gradient centrifugation and attachment method were used to isolate healthy neonatal and adult mononuclear cells. FITC mAb CD14 was identified and co-cultured with 1|Ìg / ml T|Á1 and / or 500|ÌU / ml IFN|Á for 3 days. Normal T cell expression and secretion factor (RANTES), macrophage inflammatory protein 1 alpha (MIP - 1) and MIP - 1 beta in Qing. Results: The concentrations of RANTES, MIP - 1α and MIP - 1β secreted by monocytes from 14 healthy newborns and 17 normal adults were 310 ± 90,2 310 ± 6 70,182 0 ± 75 0 and 75 0 ± 2 10,25 80 ± 12 80, 1910 ± 4 60 pg / ml. Tα1 and / or IFNα markedly upregulated RANTES secreted by monocytes. Both of them were synergistic. MIP - 1α was up - regulated by Tα1 and MIP - 1β in neonatal monocytes was upregulated by IFNα. The above-mentioned treatment on neonatal mononuclear cells than adults to regulate the more obvious. CONCLUSION: The ability of neonatal monocytes to secrete RANTES and MIP - 1α is significantly lower than that of adults. Tα1 and / or IFNα upregulate three C-C chemokines secreted by both neonatal and adult monocytes, potentially reducing the susceptibility of neonatal monocytes to HIV infection.