论文部分内容阅读
目的:探讨CTLA4Ig与CD40Ig双基因局部转染对异种移植肾Bcl-2/Bax的影响,探讨其诱导免疫耐受的机制。方法:以PcDNA3.1(+)-CTLA4Ig和PcDNA3.1(+)-CD40Ig为载体,通过脂质体lipo2000将CTLA4Ig与CD40Ig双基因转入豚鼠肾脏,以豚鼠为供者,SD大鼠为受者,行异种肾移植手术。将豚鼠到SD大鼠的异种肾移植模型分为pcDNA3.1空载体组(第1组)、CD40Ig基因局部转染组(第2组)、CTLA4Ig基因局部转染组(第3组)和CD40Ig、CTLA4Ig双基因局部转染组(第4组)。Western blot检测移植肾HA-CTLA4Ig、HACD40Ig蛋白表达和移植肾Bcl-2、Bax的表达情况,观察移植肾病理改变。结果:术后第5天,第4组移植肾淋巴细胞浸润明显少于第2组和第3组,第4组移植肾Bcl-2蛋白表达明显升高(P<0.05),而Bax蛋白表达则显著降低(P<0.05)。结论:CTLA4Ig与CD40Ig双基因局部转染供肾可降低Bax表达,上调Bcl-2表达,诱导移植肾免疫耐受。
Objective: To investigate the effect of CTLA4Ig and CD40Ig double gene transfection on the Bcl-2 / Bax in xenografted kidneys and to explore the mechanism of its induction of immune tolerance. Methods: The pcDNA3.1 (+) - CTLA4Ig and PcDNA3.1 (+) - CD40Ig vector was used to transfer the CTLA4Ig and CD40Ig double genes into guinea pig kidney via lipo2000. People, all kinds of kidney transplant. The xenograft model of guinea pig to SD rats was divided into pcDNA3.1 empty vector group (group 1), CD40Ig gene local transfection group (group 2), CTLA4Ig gene local transfection group (group 3) and CD40Ig , CTLA4Ig double gene transfection group (group 4). Western blot was used to detect the expression of HA-CTLA4Ig and HACD40Ig in renal allografts and the expression of Bcl-2 and Bax in renal allografts. The pathological changes of renal allografts were observed. Results: On the fifth postoperative day, the infiltration of lymphocytes in the fourth group was significantly less than that of the second and third groups. The expression of Bcl-2 protein in the fourth group was significantly increased (P <0.05), while the expression of Bax protein Was significantly lower (P <0.05). Conclusion: Local delivery of CTLA4Ig and CD40Ig double gene can decrease Bax expression and up-regulate Bcl-2 expression, and induce renal allograft tolerance.