牛、马、猪、羊的胰岛素仅A链的8、9、10氨基酸残基有差别。其差别是:牛胰岛素:丙~8、丝~9、缬~(10);马胰岛素:苏~8、甘~9、异亮~(10);猪胰岛素:苏~8、丝~8、异亮~(10);羊胰岛素:丙~8、甘~9、缬~(10);其余皆为中性氨基酸。作者利用恒组分洗脱系统,按逆相高效液相色谱法将这四种胰岛素进行了分离。 Cattle, horses, pigs, sheep insulin only A chain 8,9,10 amino acid residues are different. The difference is: bovine insulin: C ~ 8, silk ~ 9, Valerian ~ 10; horse insulin: Su ~ 8, Gan ~ 9, Iso-light ~ (10); sheep insulin: C ~ 8, Gan ~ 9, Valerian ~ (10); the rest are neutral amino acids. The authors used a constant-component elution system to separate these four types of insulin by reverse-phase high-performance liquid chromatography.