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目的 :探讨纯化日本血吸虫 31 /32kDa抗原 (Sj31 /32 )诊断的特异性、敏感性及疗效考核价值。方法 :利用纯化Sj31 /32与可溶性虫卵抗原 (SEA)ELISA方法检测急、慢性日本血吸虫病患者血清。结果 :急、慢性血吸虫病患者血清抗Sj31 /32抗体检出率为 1 0 0 %和 95% ,与正常人、肝吸虫和肺吸虫病患者血清未出现交叉反应 ;抗SEA抗体检出率为 97.4 %和 90 % ,与正常人和肠道吸虫病患者有不同程度的交叉反应。血吸虫病人治疗后 3个月、半年和 1年抗Sj31 /32抗体的阴转率分别为 52 .5%、72 .5%和 82 .5% ,而抗SEA抗体的阴转率则分别为 1 5%、37.5%和 50 %。结论 :Sj31 /32抗原诊断血吸虫病敏感性高、特异性强 ,并且具有较好的疗效考核价值 ,可用于血吸虫病现场和临床应用
Objective: To investigate the specificity, sensitivity and therapeutic value of the purification of 31 / 32kDa antigen (Sj31 / 32) of Schistosoma japonicum. Methods: The serums of patients with acute and chronic schistosomiasis japonica were detected by ELISA method of purified Sj31 / 32 and soluble egg antigen (SEA). Results: The detection rates of serum anti-Sj31 / 32 antibodies in acute and chronic schistosomiasis patients were 100% and 95%, respectively. No cross-reaction was found with the serums of normal, hepatic and paragonimiasis patients. The detection rate of anti-SEA antibodies was 97.4% and 90%, with normal people and intestinal fluke patients have different degrees of cross-reaction. The negative conversion rates of anti-Sj31 / 32 antibodies in schistosomiasis patients for three months, six months and one year after treatment were 52.5%, 72.5% and 82.5%, respectively, while the anti-SEA antibody negative conversion rates were 1 5%, 37.5% and 50%. Conclusion: Sj31 / 32 antigen is highly sensitive and specific for the diagnosis of schistosomiasis, and it has good value in curative effect evaluation and can be used in the field and clinical application of schistosomiasis