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从我省两名经性途径感染艾滋病的病人中采血，分离外周血单核细胞（ＰＢＭＣｓ），将其与正常人ＰＢＭＣｓ共培养分离人免疫缺陷病毒（ＨＩＶ），３周后检测上清ＨＩＶ－１ｐ２４抗原（ＥＬＩＳＡ法）超过阈值。将共培养第四周细胞和上清分别感染Ｈ９细胞（Ｔ细胞淋巴瘤传代细胞），一周后检测ＨＩＶ－１ｐ２４抗原，证明有病毒生长。用ＨＩＶ－１ＥＮＶ基因引物的套式聚合酶链反应（ＮｅｓｔｅｄＰＣＲ）证实，两株新分离的病毒为ＨＩＶ－１型。两株病毒分别命名为ＧＤ－１、ＧＤ－２，其中ＧＤ－１毒株经序列分析为Ｅ亚型。此毒株在Ｈ９细胞中连续传代后，仍能产生明显的细胞病变，并保持较高的滴度
Peripheral blood mononuclear cells (PBMCs) were collected from two AIDS-infected patients in our province and co-cultured with normal human PBMCs to isolate human immunodeficiency virus (HIV). Three weeks later, the supernatant HIV- The 1p24 antigen (ELISA method) exceeded the threshold. H9 cells (T cell lymphoma passage cells) were infected with the fourth week of co-culture of the cells and the supernatant, respectively, and HIV-1 p24 antigen was detected one week later, demonstrating the growth of the virus. The nested PCR of the HIV-1 ENV gene primers (NestedPCR) confirmed that the two newly isolated viruses were of the HIV-1 type. The two viruses were named as GD-1 and GD-2 respectively, of which the GD-1 strain was sequenced as E subtype. After continuous passage of this strain in H9 cells, it still produced obvious cytopathic effect and maintained high titer