以高脂饲料饲喂昆明种小鼠构建高血脂小鼠模型,灌胃给予高、中、低剂量(0.4、0.2和0.1 g/kg)蚯蚓冻干粉,每天一次,同时饲喂高脂饲料,共饲养10周。提取小鼠肝脏总RNA,应用逆转录-聚合酶链反应(RT-PCR)技术检测胆固醇7α-羟化酶(CYP7α1)的mRNA水平。结果表明,实验组小鼠的CYP7α1 mRNA相对含量显著高于高脂饲料组小鼠,提示蚯蚓冻干粉能够降低高血脂小鼠血浆胆固醇水平可能与上调肝CYP7α1 mRNA的表达有关。 Kunming mice were fed with high-fat diet to establish hyperlipidemic mouse model. The high, medium and low doses of earthworm lyophilized powder (0.4, 0.2 and 0.1 g / kg) , Feeding a total of 10 weeks. Total RNA was extracted from liver of mice, and the mRNA level of cholesterol 7α-hydroxylase (CYP7α1) was detected by reverse transcription-polymerase chain reaction (RT-PCR). The results showed that the relative content of CYP7α1 mRNA in experimental mice was significantly higher than that in high fat diet mice, suggesting that earthworm lyophilized powder can reduce the plasma cholesterol in hyperlipidemic mice may be related to the upregulation of the expression of liver CYP7α1 mRNA.