应用rep PCR(repetitiveelement basedpolymerasechainreaction)分子指纹技术,对75个四川稻瘟病菌菌株进行了DNA分子指纹扩增和聚类分析。结果表明,供试菌株基因组用Pot2 1和Pot2 2引物共扩增出29条DNA谱带,其中14条为多态性DNA带,各菌株分别扩增出4到17条不等的带谱;经聚类分析,在0 19连锁距离点,所有供试菌株分为7个遗传宗亲群组,其中第5宗亲群和7宗亲群分别为优势宗亲群。供试菌株传统小种划分的致病型与遗传宗亲群之间没有明显的相关性。 Using rep PCR (repetitiveelement based polymerase chain reaction) molecular fingerprinting, DNA fingerprinting and cluster analysis of 75 M. grisea isolates were carried out. The results showed that a total of 29 DNA bands were amplified by Pot2 1 and Pot2 2 primers, 14 of them were polymorphic DNA bands, and 4 to 17 bands were amplified from each strain. According to the cluster analysis, all the tested strains were divided into 7 genetic clan groups at the distance of 0 19 linkage, of which 5 and 7 were the dominant clan. There was no significant correlation between the pathogenicity and genetic ancestry groups of the traditional race of the tested strains.