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目的 观察叶酸(FA)、维生素B6(W6)、维生素B12(VB12)对同型半胱氨酸(Hcy)上调内皮细胞血管细胞粘附分子-1(VCAM-1)表达抑制作用.方法 将对数生长期大鼠主动脉内皮细胞随机分为5组,空白对照组、1 mmol/L Hcy组、抑制组I(1 mmol/L Hcy+5 μmol/L FA)、抑制组Ⅱ(1 mmol/L Hcy+5 μmol/L FA+5 nmol/LVB12)、抑制组HI(1 mmol/L Hcy+5 μmol/L FA+0.1 μmol/L VB6+5 nmol/L VB12)、逆转录聚合酶链反应(RT-PCR)检测血管细胞粘附分子VCAM-1的表达.结果 与空白对照组比较,1 mmol/L Hcy明显上调核转录因子Kb(NF-Kb)和VCAM-1 mRNA的表达,内皮-单核细胞粘附率由6.03%增加到59.04%(P<0.05);与1 mmol/LHcy组比较,3个抑制组NF-Kb(F=11.547,P=0.000)、VCAM-1 mRNA(F=94.014,P=0.000)的表达均明显下调,抑制组Ⅰ、Ⅱ、Ⅲ的内皮-单核细胞的粘附率分别为44.98%,27.23%,10.83%,均明显低于1 mmol/L Hcy组(P<0.05).结论 叶酸、VB6、VB12均可抑制NF-Kb、VCAM-1 mRNA的表达,减少内皮-单核细胞的粘附,缓解Hcy对血管内皮细胞的损伤作用,且三者同时使用效果更好.“,”Objective To observe whether folic acid(FA) ,VB6 and VB12 have effects of inhibiting vascular cell ahe-sion molecule-1 (VCAM-1) expression in cultured endothlial cells in vitro. Methods Cultured rat aortic endothelial cells were inoculated in vitro and divided into five groups:control group, 1 mmol/L homocysteine(Hey) group,inhibiting group Ⅰ(1 mmol/L Hcy + 5 μmol/L FA), inhibiting group Ⅱ(1 mmol/L Hey + 5 μmol/L FA + 5 nmol/L VB12), inhibiting group Ⅲ(1 mmoL/L Hcy + 5 Ⅲmol/L FA + 0. 1 Ⅲmol/L VB6 + 5nmol/L VB12). The expression of VCAM-1 mRNA in rat aortic endothelial cella induced by 1 mmol/L Hey for 6 hours was assayed by RT-PCR. Results The expression level of NF-KB protein and VCAM-1 mRNA was up-regulated in the cultured rat aortic endothelial cells induced by 1.0 mmoL/L Hey for 6hr and 24hr respectively,and the rate of endotheliocyte-monocyte adhesion increased significantly. The effects in-duced by Hey could be inhibited significantly by FA,VB6 and VB12 ,and the inhibitory effects adding FA,VB6 and VB12 together were better than other treatments. Conclusion FA,VB6 and VB12 may alleviate the injuries induced by Hey in cultured endothelial cells in vitro through inhibiting the expression of NF-KB protein and VCAM-1 mRNA and decreasing the rate of endotheliocyte-monocyte adhesion.