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目的：实现微量标本测定脑组织Na＋、K＋和水的含量。方法：SD大鼠脑梗死模型，用B－D18G腰穿针自制微量取样器，重新设定火焰光谱分析仪测定程序实现微量测定。结果：微量脑标本湿重仅1．93±0.33mg，干重O．43±0．08mg，梗死区Na＋542．74±135mEq／kg；K＋163．35±116．6mEq／kg。结论：本文用单一微样即可完成Na＋、K＋和水的微量测定，其结果与大标本测定相一致；本方法为今后脑微小区域水电解质分析奠定了基础。其研究结果表明，脑梗死早期脑水肿的形成与钠离子内流及伴随其移动的水的积聚有关。
Objective: To determine the content of Na +, K + and water in brain tissue by micro-specimens. Methods: The cerebral infarction model of SD rats was established by B-D18G lumbar puncture needle home-made micro-sampler, and then the determination program of flame spectrum analyzer was re-set to realize the micro assay. Results: The micro-brain specimens wet weight was only 1.93 ± 0.33mg, dry weight O. 43 ± 0.08 mg, infarct area Na + 542.74 ± 135 mEq / kg; K + 163.35 ± 116.6 mEq / kg. CONCLUSION: In this paper, a single micro sample to complete the determination of Na +, K + and water, the results consistent with the determination of large specimens; this method for the future subtle regional water electrolyte analysis laid the foundation. The results show that the formation of cerebral edema in early stage of cerebral infarction is related to the influx of sodium ion and the accumulation of water with its movement.