利用２，４－Ｄ和６－ＢＡ２种外源激素对烟草未授粉子房单倍体进行了直接途径和间接途径的诱导。结果表明，２，４－Ｄ能诱导子房通过间接途径形成胚状体；６－ＢＡ浓度为２２２μｍｏｌ·Ｌ－１和４４４μｍｏｌ·Ｌ－１时，胚状体的形成为间接途径，浓度为８８９μｍｏｌ·Ｌ－１时则为直接途径。在一定用量范围内，２种激素都有随浓度的增加胚状体诱导率提高的趋势。２种激素不同浓度配合使用，其诱导途径有一定差异，当２，４－Ｄ浓度为２２６μｍｏｌ·Ｌ－１，６－ＢＡ浓度为１７７８μｍｏｌ·Ｌ－１时胚状体的诱导率最高，达８６６７％ Induction of non-pollination ovary haploid by 2,4-D and 6-BA2 kinds of exogenous hormones was carried out by direct and indirect ways. The results showed that 2,4-D induced the formation of embryoid bodies in the ovary via indirect pathway. When the concentration of 6-BA was 222μmol·L-1 and 444μmol·L-1, the formation of embryoid body was an indirect pathway , The concentration of 889μmol·L-1 is the direct way. Within a certain dosage range, the two hormones have the tendency of increasing the induction rate of embryoid body with increasing concentration. When the concentration of 2,4-D was 226μmol·L-1,6-BA, the induction rate of embryoid body was 1778μmol·L-1 The highest, up to 86  67% 