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目的通过小鼠支气管哮喘模型,探讨芪仙清鸣颗粒治疗支气管哮喘的作用及其机制。方法选取30只健康雌性BALB/c小鼠,随机分为空白对照组(对照组)、支气管哮喘模型组(哮喘组)和芪仙清鸣颗粒治疗组(治疗组)。采用卵白蛋白致敏法建立支气管哮喘小鼠模型,造模成功后予药物处理2周;采用苏木精-伊红(H-E)染色观察病理组织学变化,过碘酸雪夫(PAS)染色检测气道黏液分泌情况,酶联免疫吸附试验(ELISA)检测血清白细胞介素(IL)-4水平,免疫组织化学法检测人第10号染色体缺失的磷酸酶和张力蛋白同源的基因(PTEN)蛋白表达情况。PTEN蛋白表达以其阳性区域的面积(Area)/累积光密度值(IOD值)表示。结果对照组小鼠肺组织小支气管无炎性细胞浸润、气道无增厚,气道无明显黏液分泌;哮喘组小鼠肺组织呈明显炎性浸润、气道明显增厚,气道黏液分泌显著增加;治疗组小鼠肺组织炎性浸润较哮喘组缓解,气道略有增厚,气道黏液分泌明显减少。哮喘组小鼠的血清IL-4水平为(6.34±4.71)pg/mL,显著高于对照组的(2.28±1.10)pg/mL(P=0.016);治疗组小鼠的血清IL-4水平为(2.82±1.27)pg/mL,显著低于哮喘组(P=0.035)。哮喘组小鼠肺组织PTEN的表达量为(0.06±0.04)IOD/Area,显著低于对照组的(0.15±0.10)IOD/Area(P=0.011);治疗组小鼠肺组织PTEN的表达量为(0.11±0.04)IOD/Area,显著高于哮喘组(P=0.013)。结论芪仙清鸣颗粒能显著抑制支气管哮喘小鼠气道炎性反应,改善病理损伤。选择性上调PTEN可能是其治疗支气管哮喘的作用机制之一。
Objective To investigate the effect and mechanism of Qixian Qingming granule in treating bronchial asthma by mouse bronchial asthma model. Methods Thirty healthy female BALB / c mice were randomly divided into blank control group (control group), bronchial asthma model group (asthma group) and Qixian Qingming granule treatment group (treatment group). A mouse model of bronchial asthma was established by ovalbumin sensitization. After successful modeling, the mice were treated with drugs for 2 weeks. The hematoxylin and eosin (HE) staining was used to observe the histopathological changes and the detection of gas by periodic acid-Schiff (PAS) Serum interleukin (IL) -4 levels were detected by enzyme-linked immunosorbent assay (ELISA) and PTEN protein (human phosphatase and tensin homology deleted on chromosome 10) by immunohistochemistry Express the situation. PTEN protein expression is expressed as the area / accumulated optical density value (IOD value) of its positive area. Results The mice in the control group showed no bronchial infiltration in the lung tissue, no airway thickening and no obvious mucus secretion in the airway. The lung tissue of the asthmatic group showed obvious inflammatory infiltration, airway thickening, airway mucus secretion The infiltration of lung tissue in the treatment group was relieved compared with the asthma group, the airway was slightly thicken and the airway mucus secretion was significantly reduced. The level of IL-4 in asthmatic mice was (6.34 ± 4.71) pg / mL, which was significantly higher than that of control group (2.28 ± 1.10) pg / mL (P = 0.016) (2.82 ± 1.27) pg / mL, significantly lower than the asthma group (P = 0.035). The expression of PTEN in lung tissue of asthmatic mice was (0.06 ± 0.04) IOD / Area, which was significantly lower than that of the control group (0.15 ± 0.10) IOD / Area (0.11 ± 0.04) IOD / Area, significantly higher than the asthma group (P = 0.013). Conclusion Qixian Qingming granule can significantly inhibit airway inflammatory response in mice with bronchial asthma and improve pathological injury. Selective upregulation of PTEN may be one of the mechanisms of its treatment of bronchial asthma.