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目的 :建立快速测定血清中苯巴比妥、苯妥英和卡马西平浓度的方法。方法 :色谱柱DiamonsilC18(4 .6mm× 2 5 0mm ,5 μm) ,流动相甲醇∶水∶乙腈 (4 0∶4 5∶15 ) ,流速1.2ml·min-1,检测波长 2 4 0nm ,内标硝西泮 ,柱温 4 0℃。结果 :提取回收率为 84 .4～ 89.2 % ,日内和日间RSD均 <7.9% ;标准曲线相关性良好。对 10 4例患者作血清浓度监测 ,临床效果满意。结论 :本方法可作为苯巴比妥、苯妥英和卡马西平的血药浓度监测的常规方法。
Objective: To establish a rapid method for the determination of phenobarbital, phenytoin and carbamazepine in serum. Methods: The chromatographic column Diamonsil C18 (4.6 × 250 mm, 5 μm) was used. The mobile phase consisted of methanol: water and acetonitrile (40:15:15), the flow rate was 1.2 ml · min- Standard nitrazepam, column temperature 4 0 ℃. Results: The recoveries ranged from 84.4% to 89.2%. The intra-day and inter-day RSDs were both <7.9%. The calibration curve was well correlated. Serum concentrations of 104 patients were monitored, with satisfactory clinical results. Conclusion: This method can be used as a routine method for the monitoring of plasma concentrations of phenobarbital, phenytoin and carbamazepine.