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采用反相ＨＰＬＣ法测定卷烟烟气总粒相物中烟碱的含量，流动相为水：Ｈ３ＰＯ４ － ＫＨ２ＰＯ４（５０：５０）缓冲液，检测波长λ＝２６２ｎｍ ，线性范围：７ ～１００ｍｇ／５０ｍｌ。实验证明，该方法的准确度高，精密度好，可作为检测烟碱的可靠方法
The content of nicotine in the total particulate matter of cigarette smoke was determined by reverse phase HPLC. The mobile phase was water: H3PO4 - KH2PO4 (50:50) buffer, the detection wavelength was λ = 262nm and the linear range was 7 ~ 100mg / 50ml. Experiments show that the method has high accuracy and good precision and can be used as a reliable method for testing nicotine