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目的探讨谷胱甘肽Ｓ－转移酶（ＧＳＴ）在大肠癌早期诊断中的价值及意义。方法应用免疫组织化学ＬＳＡＢ法，检测５４例大肠癌，１７例大肠腺瘤和３１例大肠正常粘膜中ＧＳＴ－ｐｉ的表达状态及分布特点。并应用免疫电镜技术对６例新鲜癌组织进行ＧＳＴ－ｐｉ的定位观察。结果ＧＳＴ－ｐｉ在大肠癌、腺瘤及正常粘膜中的表达阳性率分别为８１．４８％，４７．１０％和１２．９％，在不典型增生的腺瘤中阳性率高达８５．７％。大肠癌组、腺瘤组中ＧＳＴ－ｐｉ的表达均高于正常粘膜（Ｐ＜０．０１，Ｐ＜０．０５），而大肠癌与不典型增生腺瘤组比较差异无显著性（Ｐ＞０．０５）。结论ＧＳＴ－ｐｉ的高表达与大肠癌的发生有密切关系；ＧＳＴ－ｐｉ在不典型增生的腺瘤中的过量表达，可作为早期癌变的参考诊断指标
Objective To investigate the value and significance of glutathione S-transferase (GST) in the early diagnosis of colorectal cancer. Methods Immunohistochemical LSAB method was used to detect the expression and distribution of GST-pi in 54 cases of colorectal cancer, 17 cases of large intestine adenoma and 31 cases of normal colorectal mucosa. Immunoelectron microscopy was used to observe the GST-pi in 6 fresh cancer tissues. Results The positive rates of GST-pi in colorectal cancer, adenoma and normal mucosa were 81.48%, 47.10% and 12.9%, respectively. The positive rate of GST-pi in atypical hyperplasia was as high as 85.7%. . The expression of GST-pi in colorectal cancer and adenoma was higher than that in normal mucosa (P<0.01, P<0.05), but there was no significant difference between colorectal cancer and dysplasia (P>0.05). 0.05). Conclusion The high expression of GST-pi is closely related to the occurrence of colorectal cancer. Overexpression of GST-pi in atypical hyperplasia can be used as a reference diagnostic indicator for early canceration.