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以氯碘羟喹的同系物8-羟喹与Fe~(3+)相互作用使脑匀浆脂质过氧化,研究黄芪桂枝五物汤对氧化刺激的抑制作用。 选7周龄体重30～40g的ddY雄性小鼠进行以下实验:①脑匀浆脂质过氧化反应:取脑,用100mM磷酸缓冲液(pH7.5)制成脑匀浆。标准反应液为含ling蛋白质/ml脑匀浆、100μM 8-羟喹、100μM FeCl_3的反应液(100mM磷酸缓冲液pH7.5),于37℃恒温
The lipid peroxidation of brain homogenate was induced by the interaction of the homologue of clioquinol with 8-hydroxyquinoline and Fe 3+ to study the inhibitory effect of Huangqi Guizhi Wuwu Decoction on oxidation stimulation. The following experiment was performed on ddY male mice 7 days old weighing 30-40 g: 1 Lipid peroxidation reaction of brain homogenate: The brain was taken and brain homogenate was made with 100 mM phosphate buffer (pH 7.5). The standard reaction solution was a reaction solution containing ling protein/ml brain homogenate, 100 μM 8-hydroxyquinoline, and 100 μM FeCl 3 (100 mM phosphate buffer pH 7.5), and was thermostated at 37°C.