论文部分内容阅读
利用色氨酸残基作为内源荧光探针在膜模拟剂（各种表面活性剂）和不同变性剂中对大肠杆菌碱性磷酸酶（AP）的构象变化进行了系统的研究。通过测定在不同变性时间下盐酸肌浓度对荧光强度的影响以及荧光强度随pH有规律的变化，进一步证实了该蛋白质变性过程中形成较稳定中间态的结论。
The conformational changes of Escherichia coli alkaline phosphatase (AP) were systematically investigated using tryptophan residues as endogenous fluorescent probes in membrane-mimicking agents (various surfactants) and different denaturants. The effect of hydrochloric acid concentration on the fluorescence intensity at different denaturation times and the regularity of the fluorescence intensity with pH were further confirmed. The conclusion is that the formation of a more stable intermediate state during the denaturation of the protein.