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为探讨钉螺抗原作免疫原获得抗日本血吸虫感染的可能性,取不含吸虫的钉螺头足部及消化腺分别用洛克氏溶液匀浆,冻融,离心取上清液,其蛋白质含量分别为720μg/ml及780μg/ml。同法制备尾蚴抗原,蛋白质含量370μg/ml。做如下试验:分5组,每组10鼠。 第一组为未处理对照组;第二组为弗氏完全佐剂对照组,0.1ml腹腔注射,隔周1次共2次;第三组为尾蚴抗原免疫对照组;第四及第五组分别为钉螺头足抗原和消化腺抗原免疫组。各种抗原分别
In order to explore the possibility that the snail antigens act as an immunogen to obtain anti-Schistosoma japonicum infection, the snail head and digestive gland not containing the trematodes were homogenized with Lock’s solution, frozen and thawed, and the supernatant was centrifuged to obtain the protein content of 720 μg / ml and 780 μg / ml. The same method to prepare cercaria antigens, protein content 370μg / ml. Do the following test: divided into 5 groups, each group of 10 rats. The first group was untreated control group; the second group was complete Freund’s adjuvant control group, 0.1ml intraperitoneal injection once every other week for a total of 2 times; the third group was cercaria antigen immunization control group; the fourth and fifth group Respectively, screw capitatum antigen and digestive gland antigen immune group. Various antigens are respectively