人浸润型淋巴瘤(简称TIL)细胞,经过一定时间体外培养后,保留约25％的细胞,定期加入适宜的培养液进行繁殖。另将一定量的TIL细胞转移到大培养瓶内,加入培养液,定期分瓶繁殖,并补充入原培养瓶内的培养液或细胞以促进或激活其中的TIL细胞,混合后置于37℃5％CO_2培养箱中。原培养瓶中pH应小于7,细胞密度为1×10~6个/ml或以上。按上述方法培养的细胞上清液中含有白介素-2的重组体,TIL细胞至少可达2×10~(11)个。该方法使原一个周期的培养所需 Human infiltrating lymphoma (TIL) cells, after a certain period of in vitro culture, retain about 25% of the cells, regularly adding appropriate culture medium for breeding. In addition, a certain amount of TIL cells are transferred to a large culture flask, and the medium is added into the culture medium to multiply the bottle regularly. The culture medium or cells in the original culture bottle are replenished to promote or activate the TIL cells therein, 5% CO 2 incubator. The original culture flask pH should be less than 7, the cell density of 1 × 10 ~ 6 / ml or more. The supernatant of the cell cultured in the above method contains the recombinant interleukin-2, TIL cells at least up to 2 × 10 ~ (11). This method makes the original one cycle of culture required