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以4个引种果蔗品种幼苗叶片为试验材料,采用人工模拟低温环境对其进行低温胁迫,测定各品种叶片的相对电导率,丙二醛(MDA)、可溶性糖、可溶性蛋白和脯氨酸含量。结果表明,随处理时间的延长,4个引种果蔗品种幼苗叶片的相对电导率一直保持升高的趋势;MDA含量呈先显著升高后降低的趋势,分别在第7天和第10天达到最大值,顺序均为川蔗26号>拔地拉>闽引黄皮果蔗>桂果蔗1号。低温胁迫下,可溶性糖、可溶性蛋白质、脯氨酸含量呈上升趋势。随着低温处理时间的延长,叶片中可溶性糖和可溶性蛋白质含量明显增加。低温处理初期,脯氨酸含量急剧上升,随着处理时间延长脯氨酸含量开始缓慢降低,但整个过程中脯氨酸含量始终保持在较高水平。各项指标测定结果显示,4个引种果蔗品种的耐寒性表现为桂果蔗1号>闽引黄皮果蔗>拔地拉>川蔗26号。
Four seedlings of introduced sugarcane varieties were used as test materials, and their relative electric conductivity, malondialdehyde (MDA), soluble sugar, soluble protein and proline content were measured under artificial simulated low temperature environment under low temperature stress. . The results showed that with the prolongation of treatment time, the relative electrical conductivity of seedling leaves of four introduced sugarcane varieties kept a rising trend. The content of MDA increased first and then decreased, reaching the highest on the 7th day and the 10th day respectively The maximum value was in the order of Sichuan Cane 26> Putila> Fujian Huangpi cane> Gui cane No.1. Under low temperature stress, soluble sugar, soluble protein and proline content increased. With the extension of low temperature treatment, soluble sugar and soluble protein content in leaves increased significantly. The content of proline in the early stage of low temperature treatment increased sharply, and the proline content began to decrease slowly with the prolongation of treatment time. However, the content of proline in the whole process kept at a high level. The results of various indexes showed that the cold tolerance performance of the four introduced varieties of sugarcane was Gui Gua No. 1> Pui Huangxian> sugarcane> Putila> No.28.