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目的探讨染矽小鼠血液及支气管肺泡灌洗液(BALF)中涎液化糖链抗原-6(KL-6)含量与肺纤维化关系。方法雌雄各半的60只C57BL/6小鼠,随机分为染矽组和对照组,染矽组经气管滴入二氧化硅,对照组滴入生理盐水;各组小鼠于染硒后30、45、60、75、90 d分批处死,收集血液和BALF检测KL-6含量;肺组织切片行Masson胶原染色,显微镜评估肺纤维化程度。结果染矽组小鼠染硒45~90 d肺组织出现胶原沉积;与染矽45 d小鼠比较,染矽60、75、90 d小鼠肺纤维化评分值均显著增加(P<0.01);染矽60、75、90 d小鼠血液KL-6含量明显高于对照组和染矽45 d小鼠(P<0.01);染矽75、90 d小鼠BALF中KL-6含量明显高于对照组和染矽60 d小鼠(P<0.01);小鼠血液及BALF中KL-6含量与纤维化评分值呈正相关(r=0.866、0.737,P<0.01)。结论小鼠染矽45~90 d出现肺纤维化,血液及BALF中KL-6含量与肺纤维化程度呈正相关。
Objective To investigate the relationship between salivary liquefied glycogen antigen-6 (KL-6) and pulmonary fibrosis in the blood of mice and the bronchoalveolar lavage fluid (BALF). Methods Sixty male and 60 female C57BL / 6 mice were randomly divided into two groups: silicon group and control group. Silicone group was injected into trachea with saline group and normal saline in control group. , 45,60,75,90 d were sacrificed in batches, blood and BALF were collected to detect KL-6 content; lung tissue sections were stained with Masson collagen, and the degree of pulmonary fibrosis was evaluated by microscope. Results Compared with the 45-day-old silicone-infected mice, the lungs of mice in the silicon-exposed group exhibited collagen deposition 45-90 days after infection. Compared with the 45-day-old silicon mice, the values of pulmonary fibrosis in the mice treated with silicon for 60, 75 and 90 days increased significantly (P <0.01) (P <0.01). The content of KL-6 in the BALF of mice on day 75,90 d after silicon treatment was significantly higher than that of the control group and silicon 45 d (P <0.01). The KL-6 content in the blood and BALF was positively correlated with fibrosis score (r = 0.866,0.737, P <0.01). Conclusion Pulmonary fibrosis occurs in 45 ~ 90 d of silicon in mice, and there is a positive correlation between the content of KL-6 in blood and BALF and the degree of pulmonary fibrosis.