论文部分内容阅读
油松针叶束经离体培养形成幼苗的整个过程，可划分为三个阶段，即愈伤组织的起源与形成；根原基的发生和不定根的形成；芽的萌动与生长。发生在针叶束切口上的愈伤组织起源于形成层和韧皮部的薄壁细胞，经脱分化恢复分生组织的生理机能，并进行分裂活动形成愈伤组织。根原基主要是从愈伤组织内发生，属于愈伤组织生根类型。在愈伤组织内分化出来的分生组织结节和维管组织结节，经单向极性分裂活动形成根原基，并继续生长突破愈伤组织成为不定根。芽在愈伤组织出现后起动，生长锥进行缓慢的分裂活动，由扁圆形芽体变为长圆形，并形成初生叶原基与幼叶紧包生长锥。移植后芽生长迅速，长出数枚窄条状初生叶，不久即抽梢。
The whole process of Pinus tabulaeformis seedlings formed by in vitro culture can be divided into three stages, namely the origin and formation of callus, the occurrence of root primordium and adventitious root formation, bud germination and growth. The callus, which occurs at the incision of the needle bundle, originates in the parenchyma cells that form the layer and the phloem, dedifferentiates to restore the physiological function of the meristematic tissue, and undergoes division to form the callus. Root primordium occurs mainly from within the callus, belonging to the callus rooting type. In the callus differentiated meristematic nodules and vascular tissue nodules, the uniaxial polar division activity of the formation of root primordia, and continue to grow and break callus into adventitious roots. Buds start after the emergence of callus, growth cone slow division activities from flat round buds into oblong, and the formation of primary primordia and young leaves tightly wrapped growth cone. Buds grow rapidly after transplantation, grow several narrow strips of primary leaves, and soon that shoot tip.