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目的观察结直肠癌患者围手术期外周血 T 细胞亚群,即所有 T 细胞的表面分子(CD3)、辅助 T 细胞表面分子(CDg)、抑制 T 细胞表面分子(CD8),肿瘤坏死因子-α(TNF-α)和可溶性白细胞介素-2受体(SIL-2R)表达的动态变化。方法应用碱性磷酸酶-抗碱性磷酸酶法和酶免疫法动态检测了60例结直肠癌患者围手术期外周血 T 细胞亚群、TNF-α和 SIL-2R 的表达。结果结直肠癌患者术前 CD3、CD4细胞明显减少,CD4/CD8比值明显降低,CD8细胞明显增加,TNF-α和 SIL-2R 水平明显高于对照组;术后随时间延长,CD3、CD4细胞及 CD4/CD8比值逐渐增加,CD8细胞、TNF-α和 SIL-2R 水平逐渐降低。结论结直肠癌患者免疫功能低下,T 细胞功能受到抑制,TNF-α及 SIL-2R 水平的异常表达与肿瘤负荷有明显关系,切除肿瘤可改善患者的免疫功能。
Objective To observe peripheral blood T cell subsets in patients with colorectal cancer, that is, all T cell surface molecules (CD3), helper T cell surface molecules (CDg), suppressor T cell surface molecules (CD8), tumor necrosis factor-α Dynamic changes in the expression of (TNF-α) and soluble interleukin-2 receptor (SIL-2R). Methods The peripheral blood T lymphocyte subsets, TNF-α and SIL-2R expressions in 60 patients with colorectal cancer were detected by alkaline phosphatase-anti-alkaline phosphatase assay and enzyme immunoassay. Results The preoperative CD3 and CD4 cells were significantly reduced in patients with colorectal cancer, the ratio of CD4/CD8 was significantly lower, the number of CD8 cells was significantly increased, and the levels of TNF-α and SIL-2R were significantly higher than those of the control group. After operation, CD3 and CD4 cells were prolonged. And the CD4/CD8 ratio gradually increased, CD8 cells, TNF-α and SIL-2R levels gradually decreased. Conclusion The immune function of patients with colorectal cancer is low, and the function of T cells is inhibited. The abnormal expression of TNF-α and SIL-2R levels has a significant relationship with tumor burden. The removal of tumor can improve the immune function of patients.