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从江浙蝮蛇中分离纯化的碱性磷脂酶Ａ２在ｐＨ９．５，０．０５ｍｍｏｌ／ＬＣＨＥＳ缓冲液中，用汽相悬滴扩散的方法，获得了适用于高分辨率Ｘ射线结构分析的单晶．经Ｘ２００Ｂ面探测器分析，表明该晶体属于正交晶系，Ｐ２Ｉ２Ｉ２Ｉ空间群，晶胞参数为ａ＝９７．１３，ｂ＝１０３．６９，ｃ＝２３．２７．并收集了一套衍射数据，独立衍射点数１２００１个，数据完整度为８６．２％，Ｒｍｅｒｇｅ为０．０４５９．最高分辨率达２．０，根据分子量与晶胞体积估算，一个不对称单位含两个分子．
Purified alkaline phosphatase A2 isolated from the viper of Jiangsu and Zhejiang was used to diffuse the suspension in the pH9.5 and 0.05mmol / L CHES buffer. The single crystal suitable for high-resolution X-ray structure analysis . The X200B surface detector analysis showed that the crystal belongs to the orthorhombic, P2I2I2I space group with unit cell parameters of a = 97.13, b = 103.69, c = 23.27. A set of diffraction data was collected. The number of independent diffraction spots was 12001, the data integrity was 86.2% and Rmerge was 0.0459. The highest resolution of 2.0 , according to the molecular weight and cell volume estimation, an asymmetric unit contains two molecules.