论文部分内容阅读
目的对苯乙醇苷衍生物的辐射防护活性进行筛选。方法采用不同剂量X射线照射人皮肤成纤维细胞(Human Skin Fibroblasts,HSF),构建辐射损伤模型,以MTT法检测6种苯乙醇苷单体对HSF增殖的影响。结果与2 Gy、4 Gy和8 Gy辐射组比较,16 Gy辐射组对HSF增殖抑制作用的差异有统计学意义(P<0.01)。采用16 Gy一次性X射线照射构建辐射损伤模型,模型组与不同药物干预组在各时段比较差异均有统计学意义(P<0.01),其中毛蕊花糖苷辐射防护作用优于其他5种苯乙醇苷衍生物。X射线对HSF的增殖抑制作用与辐射剂量呈正相关。结论 X射线辐射对HSF具有明显损伤作用,而6种苯乙醇苷衍生物对其均具有一定的保护作用,其中以毛蕊花糖苷的抗辐射活性最强。
Objective To screen the radioprotective activity of phenetidine derivatives. Methods Human skin fibroblasts (HSF) were irradiated with different doses of X-rays to establish a radiation injury model. The effects of 6 phenylethanoid monomers on the proliferation of HSF were detected by MTT assay. Results Compared with 2 Gy, 4 Gy and 8 Gy radiation groups, the difference of HSF proliferation inhibition in 16 Gy radiation group was statistically significant (P <0.01). Radiation injury model was constructed by 16 Gy single-time X-ray irradiation. There was significant difference between the model group and the different drug intervention groups at different time points (P <0.01). The radiation protective effect of verbascoside was better than the other 5 phenyletin glycosides derivative. X-ray inhibition of HSF proliferation and radiation dose was positively correlated. Conclusion X-ray irradiation has a significant damage on HSF, while 6 kinds of phenyl ethyl glycoside derivatives have certain protective effect on them, of which verbascoside has the strongest anti-radiation activity.