【摘 要】
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Munc13-1 is an essential component of synaptic vesicle releasing machinery. Three rat Munc 13-1 constructs were rationally designed based on homology and functi
【机 构】
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Joint Laboratory of Huazhong University of Science and Technology and Institute of Biophysics, Colle
论文部分内容阅读
Munc13-1 is an essential component of synaptic vesicle releasing machinery. Three rat Munc 13-1 constructs were rationally designed based on homology and function, overexpressed in Escherichia coli, and purified to homogeneity with a final yield higher than 2 μg/ml of cell culture. The purified Munc 13-1 recombinant proteins had distinct oligomeric states, monodispersity and homogeneity properties.Their secondary structural contents were analyzed by the circular dichroism method, and the sedimentation coefficients of these recombinant proteins were measured by analytical ultracentrifugation. The long helical bundle-like topology of Munc 13-1 was first revealed by analysis of our data. In addition, these purified recombinant proteins provide ideal starting materials for further biochemical, biophysical, and structural studies on mammalian Munc 13 proteins.
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