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背景:臂丛撕脱损伤脊髓运动神经元的程度存在种属差异,神经元型一氧化氮合酶(Neuronalnitricoxidesynthase,nNOS)基因与运动神经元的死亡密切相关。目的:研究臂丛撕脱诱导受损运动神经元nNOS基因表达的种属差异。设计:完全随机设计,对照实验研究。地点和材料:中山大学动物实验中心提供实验所需SD大鼠、Hamster金黄地鼠、BALB/C小鼠各20只。干预:3组动物分别行右侧臂丛撕脱术,2周后取C7段脊髓进行nNOS免疫细胞化学、NADPH-d酶组织化学和中性红活细胞染色。主要观察指标:三种动物损伤侧脊髓前角nNOS阳性和存活的运动神经元数目。结果:臂丛撕脱后2周,SD大鼠和金黄地鼠损伤侧前角都可见大量强阳性的nNOS运动神经元,而BALB/C小鼠却没有此反应。统计分析显示:三组动物间nNOS阳性运动神经元数目有显著差异(F=501.502,P<0.001):SD大鼠多于金黄地鼠(55.59%与42.26%,t=5.940,P<0.001),多于BALB/C小鼠(55.59%与0%,t=51.651,P<0.001),金黄地鼠多于BALB/C小鼠(42.26%与0%,t=21.452,P0.001);三组动物间存活的运动神经元数目也有显著差异(F=110.588,P<0.001):SD大鼠多于金黄地鼠(87.29%与76.01%,t=5.252,P<0.05),多于BAL/C小鼠(87.29%与57.38%,t=19.561,P<0.001),金黄地鼠多于BALB/C小鼠(76.01%与57.38%,t=7.996,P<0.00
BACKGROUND: Brachial plexus avulsion can damage the motor neurons of the spinal cord. There is a close relationship between the neuronal nitric oxide synthase (nNOS) gene and the death of motor neurons. Objective: To study the species differences of nNOS gene expression in injured motor neurons induced by brachial plexus avulsion. Design: Completely randomized design, controlled experimental study. Venue and Materials: Animal experiment center of Sun Yat-sen University provided 20 SD rats, Hamster golden hamsters and BALB / C mice. Intervention: Three groups of animals were performed avulsion of right brachial plexus respectively. Two weeks later, C7 spinal cord was taken for nNOS immunocytochemistry, NADPH-d enzyme histochemistry and neutral red living cell staining. MAIN OUTCOME MEASURES: The number of nNOS-positive and viable motoneurons in the anterior horn of the injured spinal cord in the three animals. Results: A large number of strong nNOS motoneurons were found in the injured anterior horn of SD rats and golden hamsters 2 weeks after avulsion of the brachial plexus, whereas BALB / C mice did not respond. Statistical analysis showed that the number of nNOS positive motor neurons in three groups of animals was significantly different (F = 501.502, P <0.001): more SD rats than golden hamsters (55.59% vs 42.26%, t = 5.940, , More than BALB / C mice (55.59% vs 0%, t = 51.651, P <0.001), more than that of BALB / C mice (42.26% vs 0%, t = 21.452, P <0.001) There were also significant differences in the number of motoneurons surviving among the three groups (F = 110.588, P <0.001): more SD rats than golden hamsters (87.29% vs 76.01%, t = 5.252, P <0.05) / C mice (87.29% vs. 57.38%, t = 19.561, P <0.001), more in hamsters than BALB / C mice (76.01% vs 57.38%, t = 7.996, P <0.00