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离体培养中,经过小块茎发生途径从半夏叶柄诱导出完整植株。用实体解剖、连续切片和三维结构重组等方法观察分析后得出:小块茎顶端的叶原基由于不对称生长逐渐将顶端分生组织复盖,随后叶原基的尖端和两侧生长延缓,生长中心转移到背轴面中央,结果导致叶原基长成帽状结构。不同发育程度的雏叶和叶原基总是相互叠套在一起将顶端分生组织复盖于下面。小块茎顶端形成芽的同时,在顶芽附近分化根。 用4％海藻酸钠(w/v)包埋直径2～3mm的小块茎制成人工种子,在无菌培养基上的萌发率可达70％,在未灭菌泥炭土中约为30％。人工种皮内添加适当激素可促进萌发,而添加的营养物质作用不大。适当脱水有利于人工种子贮藏。
In vitro culture, the whole plant was induced from the petiole of Pinellia ternata via a small tuberous pathway. The results of solid dissection, serial sectioning and three-dimensional structural reorganization showed that the apical meristem gradually covered due to the asymmetric growth of leaf primordium at the tip of small tuber, and then the growth of the apical and bilateral leaf primordia was delayed and the growth center was transferred to The back of the central axis, resulting in leaf primordium into a cap-like structure. Leaflets of different developmental levels and leaf primordium are always stacked together to cover the top meristem covered below. Small tuber top forming buds at the same time, in the vicinity of the terminal bud differentiation. Artificial seeds were made from 4% sodium alginate (w / v) embedded in small tubers 2 to 3 mm in diameter with germination rates of up to 70% on sterile medium and about 30% on unsterilized peat soils . Artificial seed coat to add the appropriate hormone can promote germination, while the added nutrients little effect. Appropriate dehydration is conducive to artificial seed storage.