The complement fixation test(CFT)is a serological test that can be used to detect the presence of either specific antibody or antigen to diagnose infections,particularly with microbes that are not easily detected by culture methods.In the study,a polydimethylsiloxane(PDMS)/glass slide hybrid microfluidic device was firstly developed to manipu- The complement fixation test (CFT) is a serological test that can be used to detect the presence of either specific antibodies or antigen to diagnose infections, particularly with microbes that are not easily detected by culture methods. In the study, a polydimethylsiloxane (PDMS) / glass slide hybrid microfluidic device was developed to manipu-