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脑相关T细胞抗原的抗血清能和某些未成熟淋巴细胞特别是T细胞起反应。作者研究该血清中的组成抗体后认为它是针对存在于非T非B细胞群上的无唾液酸GM_1糖脂抗原,小鼠的天然杀伤细胞上就有这种抗原。作者用纯无唾液酸GM_1免疫家兔,获得特异性抗血清,在免疫絮状试验中它不和有关的糖脂,如GM_1GM_2,GD_(16)以及无唾液酸GM_2而仅和无唾液酸GM_1发生反应,因而具有高度的特异性。将细胞(1×10~6/0.1毫升)加入稀释为1:10倍的抗血清在室温下于Dulbecco磷酸盐缓冲盐水中孵育,再用结合荧光的羊抗兔免疫球蛋白染色,在荧光显微镜下分析。有些标本还曾用荧光激活细胞分类器(Fluoresence-activated cell sorter)核实。结果16例ALL病人末梢血和骨髓淋巴细胞染色均为阳性,其阳性率末梢
Antiserum of brain-related T-cell antigens can react with certain immature lymphocytes, especially T-cells. The authors studied the constituent antibodies in this serum and considered it to be a sialic GM1 glycolipid antigen present in non-T non-B cell populations, which is present on mouse natural killer cells. The author used pure asialo GM_1 to immunize rabbits to obtain specific antiserum, which was not related to the relevant glycolipids such as GM_1GM_2, GD_ (16) and asialoglycine GM_2 in immunofluorescence assay but only to asialo GM_1 Reacts, and therefore has a high degree of specificity. Cells (1 x 10-6 / 0.1 ml) were added to diluted 1: 10 fold antisera in Dulbecco’s phosphate buffered saline at room temperature and stained with fluorescent goat anti-rabbit immunoglobulin, Under analysis. Some specimens have also been verified using a Fluoressence-activated cell sorter. Results 16 cases of ALL patients with peripheral blood and bone marrow lymphocyte staining were positive, the positive rate of peripheral