论文部分内容阅读
为明确非特异性脂质转移蛋白基因Ta LTP5在小麦抗条锈性产生过程中的作用,本研究对该基因进行了染色体定位分析,并利用实时荧光定量PCR(q RT-PCR)技术分析了其转录表达特征。研究结果显示,小麦Ta LTP5基因定位在小麦3B染色体的短臂上,位于3BS8-0.78-1.00染色体区段;Ta LTP5在小麦与条锈菌的非亲和过程前期表达量显著升高,而在亲和过程中没有明显变化;Ta LTP5的表达受到外源水杨酸(SA)、聚乙二醇(PEG)模拟的干旱和外源脱落酸(ABA)的胁迫强烈诱导,而外源茉莉酸甲酯(Me JA)、乙烯(ET)和低温在一定程度上抑制基因的表达。研究结果表明,在小麦-条锈菌非亲和互作中,Ta LTP5可能是小麦对条锈菌的抗性产生信号转导途径的正调控因子,而且参与了小麦的非生物胁迫应答过程。本研究结果对于调控植物抗性和培育抗病品种均有重要意义。
In order to clarify the role of Ta LTP5, a non-specific lipid transfer protein gene, in the development of wheat stripe rust resistance, we analyzed the chromosomal location of this gene and analyzed its expression using qRT-PCR Transcriptional expression characteristics. The results showed that the Ta LTP5 gene was located on the short arm of chromosome 3B of wheat and located in chromosome segment 3BS8-0.78-1.00. The expression level of Ta LTP5 was significantly increased at the early stage of non-affinity of wheat and stripe rust, The expression of Ta LTP5 was strongly induced by the stress of exogenous salicylic acid (SA), polyethylene glycol (PEG) simulated drought and exogenous abscisic acid (ABA), while the exogenous jasmonic acid Methyl esters (Me JA), ethylene (ET) and hypothermia inhibit gene expression to a certain extent. The results showed that Ta LTP5 may be a positive regulator of the signal transduction pathway of resistance to stripe rust in the wheat-stripe rust non-affinity interaction and is involved in the abiotic stress response of wheat. The results of this study are of great importance for the regulation of plant resistance and breeding of resistant varieties.