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AIM:To investigate TNF-α-308 and TNFB polymorphismsin acute biliary pancreatitis (ABP) and to related them to theplasma TNF-α levels.METHODS:Genomic DNA was prepared from peripheralblood leukocytes.Genotypes and allele frequencies weredetermined in patients (n=127) and healthy controls (n=102)using restriction fragment length polymorphism analysis ofpolymerase chain reaction (PCR) products.Reading the sizeof digested bands from polyacrylamide gel demonstratedthe two alleles TNF1 and TNF2,or the two alleles TNFB1and TNFB2.RESULTS:The frequencies of TNF2 polymorphism andTNFB2 polymorphism were both similar in patients with mildor severe pancreatitis,so were in pancreatitis patients andin controls.Patients with septic shock showed a significantlyhigher prevalence of the TNF2 than those without.Nosignificant differences were found in the genotype distributionof TNF-α-308 and TNFB among different groups.PlasmaTNF-α levels did not differ significantly in ASBP patientsdisplaying different alleles of the TNF gene studied.CONCLUSION:Results indicate that TNF gene polymorphismsstudied play no part in determination of disease severity orsusceptibility to acute biliary pancreatitis;however,TNF2polymorphism is associated with septic shock from ASBP.Genetic factors are not important in determining plasmaTNF-α levels in ASBP.
AIM: To investigate TNF-α-308 and TNFB polymorphisms in acute biliary pancreatitis (ABP) and to related them to theplasma TNF-α levels. METHODS: Genomic DNA was prepared from peripheral blood leukocytes. Genotypes and allele frequencies were determined in patients (n = 127 ) and healthy controls (n = 102) using restriction fragment length polymorphism analysis of polymerase chain reaction (PCR) products. Readers the size of digested bands from polyacrylamide gel said the two alleles TNF1 and TNF2, or the two alleles TNFB1 and TNFB2.RESULTS: The frequencies of TNF2 polymorphism and TNFα polymorphism were both similar in patients with mildor severe pancreatitis, so were in pancreatitis patients and in controls. Patients with septic shock showed a significantlyhigher prevalence of the TNF2 than those without .Nosignificant differences were found in the genotype distribution of TNF-α-308 and TNFB among different groups. PlasmaTNF-α levels did not differ significantly in ASBP patientsdisplaying different alleles of the TNF gene studied. CONCLUSION: Results indicate that TNF gene polymorphisms is not playin part of determination of disease severity orsusceptibility to acute biliary pancreatitis; however, TNF2 polymorphism is associated with septic shock from ASBP. Genetic factors are not important in determining plasma TNF- [alpha] levels in ASBP.