云南辣椒疫病菌种的鉴定

来源 :云南农业大学学报 | 被引量 : 0次 | 上传用户:xiaoxin_1
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近几年,一种新的辣椒病害在云南普遍发生。作者分离、接种表明系辣椒疫病,对此病的症状作了描述。病原菌不易产生厚垣孢子;孢子囊形态变化很大:有卵形、近球形、长椭圆形或不规则形,长宽比1.5—1.9,乳突明显,没有层出现象,会脱落,带有长柄;有性生殖为异宗配合,藏卵器穿雄生;病菌能在35℃下生长,对浓度为1:8×10~6的孔雀绿不敏感,寄主范围广泛。对照有关疫霉分类资料,鉴定为Phytophthora capsici。此外,还对P. capcici利用淀粉能力及菌体的可溶性蛋白聚丙烯胺凝胶电泳作为分类特征的可行性问题作了探讨。 In recent years, a new peppery disease has occurred in Yunnan. The author isolated, inoculation showed that pepper disease, the symptoms of the disease were described. Pathogen is not easy to produce chlamydospore; sporocyst morphological changes: ovate, nearly spherical, oblong or irregular shape, aspect ratio 1.5-1.9, mastoid obvious, no layer of the phenomenon, will fall off, with Long handle; sexual reproduction is heterogeneous mating, and the ovipositor is male; the bacterium can grow at 35 ℃, insensitive to the malachite green with the concentration of 1: 8 × 10 ~ 6, with a wide host range. Phytophthora capsici was identified as Phytophthora capsici. In addition, P. capcici also discussed the feasibility of using starch ability and polyacrylamide gel electrophoresis of bacterial proteins as the classification features.
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