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目的 揭示大鼠脊髓后角内 5型代谢型谷氨酸受体 (mGluR5 )与一级传入C纤维的关系。 方法 在大鼠单侧足底注射福尔马林作为疼痛刺激 ,应用免疫组织化学技术光镜下观察疼痛对脊髓后角内mGluR5的影响 ;在大鼠蛛网膜下腔注射辣椒素 ,以毁损C纤维 ,用免疫电镜技术观察大鼠脊髓后角浅层内mGluR5阳性结构与C纤维间的超微结构关系。 结果 脊髓后角疼痛刺激侧Ⅰ～Ⅱ层内mGluR5免疫反应性较对照侧明显增强 ;在蛛网膜下腔注射辣椒素的大鼠 ,电镜下可见其后角浅层内大量变性的C纤维轴突终末与 1至多个含mGluR5的树突形成突触联系。 结论 提示脊髓后角浅层内mGluR5主要作为突触后受体参与介导和调节C纤维内对伤害性信息的传入。
Objective To reveal the relationship between type 5 metabotropic glutamate receptor (mGluR5) and primary afferent C fibers in the dorsal horn of rat spinal cord. Methods The rats were injected unilaterally with formalin as a pain stimulus. Immunohistochemistry was used to observe the effect of pain on mGluR5 in the dorsal horn of spinal cord. Capsaicin was injected into the subarachnoid space to destroy C The ultrastructure of mGluR5 positive cells and C fibers in the superficial layers of rat spinal cord were observed by immunoelectron microscopy. Results The immunoreactivity of mGluR5 in Ⅰ ~ Ⅱ layers of spinal dorsal horn pain stimulation group was significantly enhanced compared with the control group. Subcutaneous injection of capsaicin in subarachnoid space showed that many degeneration of C fiber axons The terminal forms synaptic connections with one or more dendrites containing mGluR5. The results suggest that mGluR5 mainly acts as a postsynaptic receptor in the dorsal horn of spinal cord to mediate and regulate the transmission of nociceptive information in C fibers.