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目的分析丝氨酸-苏氨酸激酶受体相关蛋白在系统性红斑狼疮(systemic lupus erythematosus,SLE)患者外周血单个核细胞中的表达水平,探讨该蛋白检测在系统性红斑狼疮病情判断中的可能应用。方法实验分为3组:SLE稳定组、SLE活动组和健康对照组,每组6例,分别收集其外周血单个核细胞,用相对和绝对定量的等量异位标签串联质谱技术对丝氨酸-苏氨酸激酶受体相关蛋白进行鉴定和相对定量分析,用Western blot技术对该蛋白的表达水平进行较大样本的独立验证。结果基于质谱分析的蛋白组学结果表明,丝氨酸-苏氨酸激酶受体相关蛋白在系统性红斑狼疮活动期患者外周血单个核细胞中表达显著下调(与正常人的相对比值为0.2906),且该蛋白的低表达为Western blot实验进一步证实。临床资料分析表明,该蛋白的表达水平与系统性红斑狼疮疾病活动指数呈负相关(r=-0.607,P<0.05)。结论丝氨酸-苏氨酸激酶受体相关蛋白可能参与SLE的发病,该蛋白有望作为系统性红斑狼疮的活动阶段特异性标志物。
Objective To analyze the expression of serine-threonine kinase receptor-related protein in peripheral blood mononuclear cells of patients with systemic lupus erythematosus (SLE) and to explore the possible application of this protein in judging the status of systemic lupus erythematosus . Methods The experiment was divided into three groups: SLE stable group, SLE active group and healthy control group, 6 cases in each group were collected peripheral blood mononuclear cells, using relative and absolute quantitative isosceles label tandem mass spectrometry for serine - Threonine kinase receptor-related protein was identified and relative quantitative analysis, the protein expression level by Western blot independent validation of larger samples. Results Proteomics results based on mass spectrometry analysis showed that the expression of serine-threonine kinase receptor-related proteins was significantly down-regulated in peripheral blood mononuclear cells from patients with active systemic lupus erythematosus (relative to normal subjects, 0.2906) and The low expression of this protein is further confirmed by Western blot. Clinical data analysis showed that the expression level of the protein was negatively correlated with SLE index (r = -0.607, P <0.05). Conclusion Serine-threonine kinase receptor-related protein may be involved in the pathogenesis of SLE, which is expected to be an active phase-specific marker of systemic lupus erythematosus.