论文部分内容阅读
目的研究拳参乙醇提取物(BRE)的免疫调节作用。方法在小鼠体外实验中,通过比色分析法检测小鼠巨噬细胞系RAW264.7细胞吞噬中性红的能力。在小鼠体内实验中,通过测定胸腺、脾脏重量并计算脏器指数;MTT法检测T淋巴细胞增殖和NK细胞活性;鸡红细胞免疫后测定小鼠血清溶血素抗体水平;ELISA法测定血清中IL-2含量。结果 BRE能够显著增强RAW264.7细胞的吞噬能力,增加正常小鼠胸腺和脾脏重量,促进T淋巴细胞增殖,增强NK细胞的细胞毒作用,上调血清溶血素水平及血清IL-2水平。结论 BRE具有一定的免疫调节作用。
Objective To study the immunomodulatory effects of ethanol extract of fistula (BRE). Methods The ability of phagocytosis of neutral red in mouse macrophage cell line RAW264.7 cells was assayed by colorimetric assay in vitro. In vivo experiments in mice, the thymus and spleen weights were measured and the organ index was calculated; T lymphocyte proliferation and NK cell activity were measured by MTT assay; Serum hemolysin antibody levels were determined after immunization of chicken erythrocytes; IL- -2 content. Results BRE could significantly enhance the phagocytosis of RAW264.7 cells, increase the weight of thymus and spleen in normal mice, promote the proliferation of T lymphocytes, enhance the cytotoxicity of NK cells, and increase the level of serum hemolysin and serum IL-2. Conclusion BRE has some immunomodulatory effects.