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分别用定量聚合酶链反应（ＰＣＲ）法和酶标法（ＥＬＩＳＡ），检测６４例慢性乙型肝炎患者血清ＨＢＶ－ＤＮＡ含量与乙肝病毒血清标记物（ＨＢＶ－Ｍ）。结果显示，ｅ抗原阳性组与ｅ抗原阴性组血清ＨＢＶ－ＤＮＡ含量分别为１０７．９１±４．１６ｃｏｐｙ／ｍｌ和１０５．８２±３．２８ｃｏｐｙ／ｍｌ，二组相比具有显著性差异（Ｐ＜０．０５）。此结果进一步证实，ｅ抗原阳性是乙肝病毒体内复制的指标；但同时也有３２．９％（２１／６４）的ｅ抗原阴性患者仍可检出ＨＢＶ－ＤＮＡ，提示ｅ抗原阴性不能认为乙肝病毒复制停止。分析认为，定量检测ＨＢＶ－ＤＮＡ含量对判断慢性乙型肝炎患者病情，估计其预后具有一定的临床参考价值。
The serum HBV-DNA levels and hepatitis B virus serum markers (HBV-M) in 64 patients with chronic hepatitis B were detected by quantitative polymerase chain reaction (PCR) and enzyme-linked immunosorbent assay (ELISA) respectively. The results showed that the serum HBV-DNA levels of e antigen positive group and e antigen negative group were 107.91 ± 4.16copy / ml and 105.82 ± 3.28copy / ml, respectively, with significant difference between the two groups (P < 0.05). The results further confirmed that e antigen positive hepatitis B virus replication in vivo indicators; but also 32.9% (21/64) of e antigen negative patients can still detect HBV-DNA, suggesting that e antigen negative can not be considered hepatitis B virus replication stop. Analysis, quantitative detection of HBV DNA content in judging the status of patients with chronic hepatitis B, it is estimated that the prognosis of a certain clinical reference value.