使用不同极性的有机溶剂分离得到了5种(石油醚、氯仿、乙酸乙酯、正丁醇、水)乌药提取物,并采用多种实验方法研究了其抗肿瘤及抗氧化活性.抗肿瘤活性研究结果显示:乌药石油醚萃取物对供试4种人癌细胞的细胞毒性最强;而HepG2细胞对乌药石油醚部位的细胞毒性最为敏感,半数抑制率(IC50)为(71.9±1.1)mg·L-1;乌药石油醚萃取物是通过诱发细胞凋亡来达到其体外抗HepG2作用的.抗氧化活性研究结果显示:乌药乙酸乙酯萃取物具有最强的体外抗氧化能力. Five kinds of petroleum ether (petroleum ether, chloroform, ethyl acetate, n-butanol and water) extracts were isolated using organic solvents with different polarities and their anti-tumor and antioxidant activities were studied by a variety of experimental methods. The results of tumor activity study showed that the petroleum ether extract of Radix Aconiti Preparatum had the strongest cytotoxicity on the four human cancer cell lines tested, while HepG2 cells were most sensitive to the cytotoxicity of Radix Botanica. The half inhibition rate (IC50) was (71.9 ± 1.1) mg · L-1. The petroleum ether extract of UFG reached its in vitro anti-HepG2 effect through the induction of apoptosis.The results of antioxidant activity showed that UFGE had the strongest in vitro anti- Oxidation ability.