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目的改进矿泉水中大肠菌群测定方法,准确评价矿泉水中大肠菌群的检出情况。方法先将500 ml矿泉水样品中加入已灭菌的0.4 g/L硫代硫酸钠1 ml中和矿泉水中氧化性消毒剂,然后按GB/T 8538-2008中矿泉水中大肠菌群多管发酵法进行检测。结果同一样品利用该法与国标法检测矿泉水中大肠菌群,经统计学检验差异有统计学意义(t=3.119,P<0.05)。结论采用改进方法测定矿泉水中大肠菌群结果准确,能真正反映出矿泉水中大肠菌群的实际数量。
Objective To improve the determination of coliform bacteria in mineral water and accurately detect the detection of coliform bacteria in mineral water. Methods The first 500 ml of mineral water samples were sterilized 0.4 g / L sodium thiosulfate 1 ml neutralization of mineral water oxidizing disinfectant, and then by GB / T 8538-2008 in the mineral water coliform bacteria multi-tube fermentation Law for testing. The results of the same sample using the method and the national standard method of detection of coliform bacteria in mineral water, the statistical test was statistically significant difference (t = 3.119, P <0.05). Conclusion The improved method for determining coliform bacteria in mineral water is accurate and can really reflect the actual quantity of coliform bacteria in mineral water.