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目前精子形态观察多采用瑞氏染色法,染色效果欠佳,影响形态观察。我们在试验中发现Feul吕en染色对精子形态染色效果好,现介绍如下。1试剂与方法1.1试剂①1mol/LHCI;②Schiff试剂;③10g/L淡绿。1.2方法将液化精液涂片,吹干。95%乙醇固定10min,1mol/LHCI(60”C)水
The current observation of sperm morphology using Wright’s staining, poor staining, morphological observation. We found in the experiment Feul Lu en dyeing staining effect on sperm morphology, are described below. 1 Reagents and methods 1.1 Reagents ① 1mol / LHCI; ② Schiff reagent; ③ 10g / L light green. 1.2 Methods Liquefaction semen smear, dry. 95% ethanol for 10 min, 1 mol / LHCI (60 "C) water