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目的 探讨微量细胞培养法在病毒性脑炎、脑膜炎病原学检测中的应用价值及北方地区病毒性脑炎、脑膜炎的病原学构成。方法 应用微量细胞培养法对 12 2例临床诊断为病毒性脑炎、脑膜炎患儿的咽分泌物、粪便及脑脊液进行病毒分离。结果 12 2例脑炎、脑膜炎患儿共取鼻咽分泌物、粪便及脑脊液标本 36 6份 ,分离到病毒 6 9株 ,总阳性率为 18 9% (6 9/36 6 ) ;其中脑脊液中分离到 10株病毒 ,阳性率为 8 2 % (10 /12 2 )。 12 2例患儿中 5 1例病毒分离阳性 ,其中肠道病毒 2 5例 ,腺病毒Ⅲ型 11例 ,单纯疱疹病毒Ⅰ型 9例 ,流感病毒 4例及巨细胞病毒 2例。结论 微量细胞培养法病毒分离具有操作简便、准确可靠、省时省物的优点 ,值得推广。
Objective To investigate the value of micro-cell culture in the etiological detection of viral encephalitis and meningitis and the etiological composition of viral encephalitis and meningitis in northern China. Methods Virus isolation was performed on the pharyngeal secretions, faeces and cerebrospinal fluid of 12 2 children with viral encephalitis and meningitis by using the method of micro-cell culture. Results A total of 366 samples of nasopharyngeal secretions, stool and cerebrospinal fluid were collected from 122 children with encephalitis and meningitis, and 69 strains were isolated, the total positive rate was 18 9% (6 9/36 6). Cerebrospinal fluid Ten viruses were isolated and the positive rate was 82% (10/122). Fifty-two cases of 12 children were positive for virus isolation, including 25 cases of enterovirus, 11 cases of adenovirus type Ⅲ, 9 cases of herpes simplex virus type Ⅰ, 4 cases of influenza virus and 2 cases of cytomegalovirus. Conclusion Micro-cell culture virus separation has the advantages of simple operation, accurate and reliable, saving time and money, it is worth promoting.