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目的观察三七对酒精性肝病大鼠肝组织NF-κB、c-Jun表达的影响。方法SD雄性大鼠连续14周给予酒精建立酒精性肝病模型,随机分为模型对照组,三七高、低剂量组和硫普罗宁组,在模型制备同时,每天下午分别灌服给药,连续14周。同时设立正常对照组,连续14周灌胃给水。免疫组化法检测肝组织中NF-κBp65/IκBα、p-IκBα、p-JNK、c-Jun蛋白的表达。结果模型对照组大鼠肝组织NF-κBp65/IκBα、p-IκBα、p-JNK/c-Jun均较正常对照组明显升高(P<0.01);三七高、低剂量组大鼠肝组织NF-κBp65/IκBα、p-IκBα、p-JNK/c-Jun表达较模型对照组明显降低(P<0.01,P<0.05)。结论三七能显著抑制肝组织中NF-κBp65/IκBα、p-JNK/c-Jun的过度表达,这可能是其有效防治酒精性肝病发生发展的重要机制之一。
Objective To observe the effect of Panax notoginseng on the expression of NF-κB and c-Jun in hepatic tissue of alcoholic liver disease in rats. Methods SD male rats were given alcohol for 14 weeks to establish model of alcoholic liver disease and were randomly divided into model control group, high and low doses of Panax notoginseng group and tiopronin group. At the same time, 14 weeks. At the same time set up a normal control group, 14 consecutive weeks of gavage water. Immunohistochemistry was used to detect the expression of NF-κBp65 / IκBα, p-IκBα, p-JNK and c-Jun in liver tissue. Results The levels of NF-κBp65 / IκBα, p-IκBα and p-JNK / c-Jun in the model control group were significantly higher than those in the normal control group (P <0.01) The expression of NF-κBp65 / IκBα, p-IκBα, p-JNK / c-Jun was significantly lower than that of the model control group (P <0.01, P <0.05). Conclusion Panax notoginseng can significantly inhibit the overexpression of NF-κBp65 / IκBα and p-JNK / c-Jun in liver tissue, which may be one of the important mechanisms for the prevention and treatment of alcoholic liver disease.