The effect of different kinds of surfactants on the extraction process of Nile Blue-I- was investigated by laser thermal lens spectrometry . It was shown that the surfactants could accelerate or decelerate the extraction rate by varying the extractive’s solubility and the interfacial tension of the extraction solution. The higher the concentration of surfactants was, the more obvious the effect. The effect of different kinds of surfactants on the extraction process of Nile Blue-I-was investigated by laser thermal lens spectrometry. It was shown that the surfactants could accelerate or decelerate the extraction rate by varying the extractive’s solubility and the interfacial tension of the extraction the higher the concentration of surfactants was, the more obvious the effect.