论文部分内容阅读
目的 观察阿片激动剂长时程作用对NO cGMP信号转导系统的影响。方法 选用iNOScDNA稳定表达的NG LNCXiNOS细胞 ,采用NOS活性和cGMP放免测定 ,Western杂交和NADPH黄递酶组化染色技术。结果阿片类药物长时程作用剂量依赖性增高胞浆相iNOS活性和胞内cGMP含量 ,药物作用强弱顺序是DPDPE >DADLE >吗啡 ,EC50 都在nmol·L-1数量级。用纳洛酮急性戒断阿片耐受细胞 ,造成酶活性和cGMP水平增加更显著。DPDPE长时程作用还引起iNOS基因表达增强和NADPH黄递酶染色阳性细胞增多。结论 提示NO cGMP信号转导系统上调可能是阿片耐受和成瘾的重要生化改变。
Objective To observe the effects of long-acting opioid agonist on NO cGMP signal transduction system. Methods NG LNCXiNOS cells stably expressing iNOS cDNA were selected. NOS activity, cGMP, Western blotting and NADPH diaphorase staining were used. Results Long-term opioid dose-dependent increase in cytoplasmic iNOS activity and intracellular cGMP content, the order of the drug effect is DPDPE> DADLE> morphine, EC50 in nmol·L-1 order of magnitude. The acute withdrawal of opiate tolerant cells with naloxone caused more significant increases in enzyme activity and cGMP levels. Long-term effects of DPDPE also cause increased iNOS gene expression and increased NADPH diaphorase-positive cells. The conclusion suggests that NO cGMP signal transduction system up-regulation may be an important biochemical alteration of opiate tolerance and addiction.