论文部分内容阅读
目的:优选葛根-黄连药对醇提的最佳提取工艺条件.方法:采用乙醇回流提取法,选择药材粒度、料液比、回流时间、乙醇浓度、回流次数为考察因素,以总黄酮、总生物碱、葛根素、盐酸小檗碱提取量为综合评价指标,采用Plackett-Burman (PB)设计对考察因素进行统计分析;运用Box-Behnken(BBD)响应面法对提取工艺参数进一步优化.结果:最佳提取工艺参数:药材粒度60目、料液比1:13、回流时间60 min、乙醇浓度75%、回流次数4次;在此条件下,总黄酮提取量120.34 mg · ml-1、总生物碱提取量为56.99 mg ·ml-1、葛根素提取量为109.63 mg ·ml-1、盐酸小檗碱提取量为39.26 mg ·ml-1,均与预测值相近.结论:采用PB设计结合BBD效应面法优选的葛根-黄连药对提取工艺合理可行.“,”Objective:To optimize the alcohol extraction process of herbal pair puerarin-berberine.Methods:Based on the etha-nol reflux extraction,the extraction quantity of total flavonoids,total alkaloid,puerarin and berberine hydrochloride were used as the e-valuation index,and the independent variables included the drug particle,ratio of solid to liquid, ethanol concentration, reflux dura-tion and reflux times. Significance analysis was evaluated by Plackett-Burman design,and then the extraction process was optimized by Box-Behnken response surface methodology.Results:The optimal extraction conditions of drug pair puerarin-berberine were as follows:the drug particle was 80 mesh,the ratio of solid to liquid was 1:13,the ethanol concentration was 75%,the reflux time was 60 min, and the reflux times was 4. Under the above conditions, the extraction quantity of total flavonoids, alkaloid, puerarin and berberine hydrochloride was 120.34,56.99,109.63 and 39.26 mg ·ml-1, respectively.Conclusion: The extraction process of herbal pair puerarin-berberine is reasonable and feasible optimized by Plackett-Burman design and Box-Behnken response surface methodology.