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羟脯氨酸是人体胶原蛋白的特有氨基酸。在胶原蛋白代谢异常时,必然反映在血羟脯氨酸的变化上,所以测定病人在治疗过程中血羟脯氨酸含量变化,对中西医结合抗肝脏纤维化治疗过程中的疗效考核,是具有一定临床意义的。我们根据目前国内供货来源,在Peter等所用自动荧光氨基酸分析法基础上,改用反相高效液相色谱法,进行了探索,取得了比较满意的结果。 层析条件 C_(18)H_(37)键合相层析柱:150×4.6mmI.D.;C_(18)H_(37)键合相预柱:50×4.6mmI.D.;荧光检测器:λ_(ex)为347nm,λ_(em)为455nm:柱温:25℃:流动相:0.03M柠檬酸三钠缓冲液(pH=7):
Hydroxyproline is a unique amino acid of human collagen. Collagen metabolism in the inevitable, is bound to reflect changes in blood hydroxyproline, so the determination of patients in the course of treatment of hydroxyproline content changes in the treatment of Integrative Medicine against liver fibrosis in the assessment of the efficacy of the process is Has some clinical significance. Based on the current domestic sources of supply, based on the automated fluorescent amino acid analysis used by Peter et al., We switched to reversed-phase high-performance liquid chromatography to explore and achieved satisfactory results. Chromatographic conditions C_ (18) H_ (37) bonded phase column: 150 × 4.6mmI.D .; C_ (18) H_ (37) bonded phase Precolumn: 50 × 4.6mmI.D .; Fluorescence detection Device: λ_ (ex) was 347 nm and λ_ (em) was 455 nm. Column temperature: 25 ° C .: Mobile phase: 0.03 M trisodium citrate buffer (pH = 7)